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Detection of single fluorescent proteins inside eukaryotic cells using two-photon fluorescence
Imaging single fluorescent proteins in a live cell is a challenging task because of the strong cellular autofluorescence. Autofluorescence can be minimized by reducing fluorescence excitation volume. Total internal reflection fluorescence (TIRF) microscopy has been routinely used to reduce excitatio...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Optical Society of America
2012
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269850/ https://www.ncbi.nlm.nih.gov/pubmed/22312586 http://dx.doi.org/10.1364/BOE.3.000340 |