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Detection of single fluorescent proteins inside eukaryotic cells using two-photon fluorescence

Imaging single fluorescent proteins in a live cell is a challenging task because of the strong cellular autofluorescence. Autofluorescence can be minimized by reducing fluorescence excitation volume. Total internal reflection fluorescence (TIRF) microscopy has been routinely used to reduce excitatio...

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Detalles Bibliográficos
Autores principales:	Hou, Ximiao, Cheng, Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Optical Society of America 2012
Materias:	Cell Studies
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269850/ https://www.ncbi.nlm.nih.gov/pubmed/22312586 http://dx.doi.org/10.1364/BOE.3.000340

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