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A versatile element for gene addition in bacterial chromosomes
The increasing interest in genetic manipulation of bacterial host metabolic pathways for protein or small molecule production has led to a need to add new genes to a chromosome quickly and easily without leaving behind a selectable marker. The present report describes a vector and four-day procedure...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273789/ https://www.ncbi.nlm.nih.gov/pubmed/22123741 http://dx.doi.org/10.1093/nar/gkr1085 |