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Error correction of microchip synthesized genes using Surveyor nuclease

The development of economical and high-throughput gene synthesis technology has been hampered by the high occurrence of errors in the synthesized products, which requires expensive labor and time to correct. Here, we describe an error correction reaction (ECR), which employs Surveyor, a mismatch-spe...

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Detalles Bibliográficos
Autores principales: Saaem, Ishtiaq, Ma, Siying, Quan, Jiayuan, Tian, Jingdong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273826/
https://www.ncbi.nlm.nih.gov/pubmed/22127863
http://dx.doi.org/10.1093/nar/gkr887