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Error correction of microchip synthesized genes using Surveyor nuclease
The development of economical and high-throughput gene synthesis technology has been hampered by the high occurrence of errors in the synthesized products, which requires expensive labor and time to correct. Here, we describe an error correction reaction (ECR), which employs Surveyor, a mismatch-spe...
Autores principales: | Saaem, Ishtiaq, Ma, Siying, Quan, Jiayuan, Tian, Jingdong |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273826/ https://www.ncbi.nlm.nih.gov/pubmed/22127863 http://dx.doi.org/10.1093/nar/gkr887 |
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