Isolation of homozygous mutant mouse embryonic stem cells using a dual selection system

Obtaining random homozygous mutants in mammalian cells for forward genetic studies has always been problematic due to the diploid genome. With one mutation per cell, only one allele of an autosomal gene can be disrupted, and the resulting heterozygous mutant is unlikely to display a phenotype. In ce...

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Detalles Bibliográficos
Autores principales: Huang, Yue, Pettitt, Stephen J., Guo, Ge, Liu, Guang, Li, Meng Amy, Yang, Fengtang, Bradley, Allan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273828/
https://www.ncbi.nlm.nih.gov/pubmed/22127858
http://dx.doi.org/10.1093/nar/gkr908

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273828/
https://www.ncbi.nlm.nih.gov/pubmed/22127858
http://dx.doi.org/10.1093/nar/gkr908

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