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Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification

Recently, we developed a simple isothermal nucleic acid amplification reaction, primer generation-rolling circle amplification (PG-RCA), to detect specific DNA sequences with great sensitivity and large dynamic range. In this paper, we combined PG-RCA with a three-way junction (3WJ) formation, and d...

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Detalles Bibliográficos
Autores principales: Murakami, Taku, Sumaoka, Jun, Komiyama, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3273829/
https://www.ncbi.nlm.nih.gov/pubmed/22127872
http://dx.doi.org/10.1093/nar/gkr909