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Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells
BACKGROUND: We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. METHODOLOGY/PRINCIPAL FINDINGS: The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming site...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275609/ https://www.ncbi.nlm.nih.gov/pubmed/22347404 http://dx.doi.org/10.1371/journal.pone.0030794 |