Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells

BACKGROUND: We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs. METHODOLOGY/PRINCIPAL FINDINGS: The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming site...

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Detalles Bibliográficos
Autores principales: Fan, Jian-Bing, Chen, Jing, April, Craig S., Fisher, Jeffrey S., Klotzle, Brandy, Bibikova, Marina, Kaper, Fiona, Ronaghi, Mostafa, Linnarsson, Sten, Ota, Takayo, Chien, Jeremy, Laurent, Louise C., Nisperos, Sean V., Chen, Gina Y., Zhong, Jiang F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275609/
https://www.ncbi.nlm.nih.gov/pubmed/22347404
http://dx.doi.org/10.1371/journal.pone.0030794

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