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The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis

The aspartate pathway of amino acid biosynthesis is essential for all microbial life but is absent in mammals. Characterizing the enzyme-catalyzed reactions in this pathway can identify new protein targets for the development of antibiotics with unique modes of action. The enzyme aspartate β-semiald...

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Autores principales: Viola, Ronald E., Faehnle, Christopher R., Blanco, Julio, Moore, Roger A., Liu, Xuying, Arachea, Buenafe T., Pavlovsky, Alexander G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE-Hindawi Access to Research 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276109/
https://www.ncbi.nlm.nih.gov/pubmed/22332000
http://dx.doi.org/10.4061/2011/352538
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author Viola, Ronald E.
Faehnle, Christopher R.
Blanco, Julio
Moore, Roger A.
Liu, Xuying
Arachea, Buenafe T.
Pavlovsky, Alexander G.
author_facet Viola, Ronald E.
Faehnle, Christopher R.
Blanco, Julio
Moore, Roger A.
Liu, Xuying
Arachea, Buenafe T.
Pavlovsky, Alexander G.
author_sort Viola, Ronald E.
collection PubMed
description The aspartate pathway of amino acid biosynthesis is essential for all microbial life but is absent in mammals. Characterizing the enzyme-catalyzed reactions in this pathway can identify new protein targets for the development of antibiotics with unique modes of action. The enzyme aspartate β-semialdehyde dehydrogenase (ASADH) catalyzes an early branch point reaction in the aspartate pathway. Kinetic, mutagenic, and structural studies of ASADH from various microbial species have been used to elucidate mechanistic details and to identify essential amino acids involved in substrate binding, catalysis, and enzyme regulation. Important structural and functional differences have been found between ASADHs isolated from these bacterial and fungal organisms, opening the possibility for developing species-specific antimicrobial agents that target this family of enzymes.
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spelling pubmed-32761092012-02-13 The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis Viola, Ronald E. Faehnle, Christopher R. Blanco, Julio Moore, Roger A. Liu, Xuying Arachea, Buenafe T. Pavlovsky, Alexander G. J Amino Acids Review Article The aspartate pathway of amino acid biosynthesis is essential for all microbial life but is absent in mammals. Characterizing the enzyme-catalyzed reactions in this pathway can identify new protein targets for the development of antibiotics with unique modes of action. The enzyme aspartate β-semialdehyde dehydrogenase (ASADH) catalyzes an early branch point reaction in the aspartate pathway. Kinetic, mutagenic, and structural studies of ASADH from various microbial species have been used to elucidate mechanistic details and to identify essential amino acids involved in substrate binding, catalysis, and enzyme regulation. Important structural and functional differences have been found between ASADHs isolated from these bacterial and fungal organisms, opening the possibility for developing species-specific antimicrobial agents that target this family of enzymes. SAGE-Hindawi Access to Research 2011 2010-12-22 /pmc/articles/PMC3276109/ /pubmed/22332000 http://dx.doi.org/10.4061/2011/352538 Text en Copyright © 2011 Ronald E. Viola et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Viola, Ronald E.
Faehnle, Christopher R.
Blanco, Julio
Moore, Roger A.
Liu, Xuying
Arachea, Buenafe T.
Pavlovsky, Alexander G.
The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title_full The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title_fullStr The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title_full_unstemmed The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title_short The Catalytic Machinery of a Key Enzyme in Amino Acid Biosynthesis
title_sort catalytic machinery of a key enzyme in amino acid biosynthesis
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3276109/
https://www.ncbi.nlm.nih.gov/pubmed/22332000
http://dx.doi.org/10.4061/2011/352538
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