Biomass production of site selective (13)C/(15)N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR

Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at different carbon sites is shown to facilitate cost-e...

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Detalles Bibliográficos
Autores principales: Thakur, Chandar S., Luo, Yiling, Chen, Bin, Eldho, Nadukkudy V., Dayie, T. Kwaku
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2011
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277826/
https://www.ncbi.nlm.nih.gov/pubmed/22124680
http://dx.doi.org/10.1007/s10858-011-9586-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277826/
https://www.ncbi.nlm.nih.gov/pubmed/22124680
http://dx.doi.org/10.1007/s10858-011-9586-1

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