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Biomass production of site selective (13)C/(15)N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR
Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at different carbon sites is shown to facilitate cost-e...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer Netherlands
2011
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277826/ https://www.ncbi.nlm.nih.gov/pubmed/22124680 http://dx.doi.org/10.1007/s10858-011-9586-1 |