Primer Extension Mutagenesis Powered by Selective Rolling Circle Amplification

Primer extension mutagenesis is a popular tool to create libraries for in vitro evolution experiments. Here we describe a further improvement of the method described by T.A. Kunkel using uracil-containing single-stranded DNA as the template for the primer extension by additional uracil-DNA glycosyla...

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Detalles Bibliográficos
Autores principales: Huovinen, Tuomas, Brockmann, Eeva-Christine, Akter, Sultana, Perez-Gamarra, Susan, Ylä-Pelto, Jani, Liu, Yuan, Lamminmäki, Urpo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280210/
https://www.ncbi.nlm.nih.gov/pubmed/22355397
http://dx.doi.org/10.1371/journal.pone.0031817

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280210/
https://www.ncbi.nlm.nih.gov/pubmed/22355397
http://dx.doi.org/10.1371/journal.pone.0031817

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