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Expression of recombinant multi-coloured fluorescent antibodies in gor (-)/trxB(- )E. coli cytoplasm

BACKGROUND: Antibody-fluorophore conjugates are invaluable reagents used in contemporary molecular cell biology for imaging, cell sorting and tracking intracellular events. However they suffer in some cases from batch to batch variation, partial loss of binding and susceptibility to photo-bleaching....

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Detalles Bibliográficos
Autores principales: Markiv, Anatoliy, Beatson, Richard, Burchell, Joy, Durvasula, Ravi V, Kang, Angray S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3280946/
https://www.ncbi.nlm.nih.gov/pubmed/22129156
http://dx.doi.org/10.1186/1472-6750-11-117