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Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis

BACKGROUND: Inflammatory bowel disease (IBD) therapies are modestly successful and associated with significant side effects. Thus, the investigation of novel approaches to prevent colitis is important. Probiotic bacteria can produce immunoregulatory metabolites in vitro such as conjugated linoleic a...

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Autores principales: Bassaganya-Riera, Josep, Viladomiu, Monica, Pedragosa, Mireia, De Simone, Claudio, Carbo, Adria, Shaykhutdinov, Rustem, Jobin, Christian, Arthur, Janelle C., Corl, Benjamin A., Vogel, Hans, Storr, Martin, Hontecillas, Raquel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283634/
https://www.ncbi.nlm.nih.gov/pubmed/22363592
http://dx.doi.org/10.1371/journal.pone.0031238
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author Bassaganya-Riera, Josep
Viladomiu, Monica
Pedragosa, Mireia
De Simone, Claudio
Carbo, Adria
Shaykhutdinov, Rustem
Jobin, Christian
Arthur, Janelle C.
Corl, Benjamin A.
Vogel, Hans
Storr, Martin
Hontecillas, Raquel
author_facet Bassaganya-Riera, Josep
Viladomiu, Monica
Pedragosa, Mireia
De Simone, Claudio
Carbo, Adria
Shaykhutdinov, Rustem
Jobin, Christian
Arthur, Janelle C.
Corl, Benjamin A.
Vogel, Hans
Storr, Martin
Hontecillas, Raquel
author_sort Bassaganya-Riera, Josep
collection PubMed
description BACKGROUND: Inflammatory bowel disease (IBD) therapies are modestly successful and associated with significant side effects. Thus, the investigation of novel approaches to prevent colitis is important. Probiotic bacteria can produce immunoregulatory metabolites in vitro such as conjugated linoleic acid (CLA), a polyunsaturated fatty acid with potent anti-inflammatory effects. This study aimed to investigate the cellular and molecular mechanisms underlying the anti-inflammatory efficacy of probiotic bacteria using a mouse model of colitis. METHODOLOGY/PRINCIPAL FINDINGS: The immune modulatory mechanisms of VSL#3 probiotic bacteria and CLA were investigated in a mouse model of DSS colitis. Colonic specimens were collected for histopathology, gene expression and flow cytometry analyses. Immune cell subsets in the mesenteric lymph nodes (MLN), spleen, blood and colonic lamina propria cells were phenotypically and functionally characterized. Fecal samples and colonic contents were collected to determine the effect of VSL#3 and CLA on gut microbial diversity and CLA production. CLA and VSL#3 treatment ameliorated colitis and decreased colonic bacterial diversity, a finding that correlated with decreased gut pathology. Colonic CLA concentrations were increased in response to probiotic bacterial treatment, but without systemic distribution in blood. VSL#3 and CLA decreased macrophage accumulation in the MLN of mice with DSS colitis. The loss of PPAR γ in myeloid cells abrogated the protective effect of probiotic bacteria and CLA in mice with DSS colitis. CONCLUSIONS/SIGNIFICANCE: Probiotic bacteria modulate gut microbial diversity and favor local production of CLA in the colon that targets myeloid cell PPAR γ to suppress colitis.
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spelling pubmed-32836342012-02-23 Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis Bassaganya-Riera, Josep Viladomiu, Monica Pedragosa, Mireia De Simone, Claudio Carbo, Adria Shaykhutdinov, Rustem Jobin, Christian Arthur, Janelle C. Corl, Benjamin A. Vogel, Hans Storr, Martin Hontecillas, Raquel PLoS One Research Article BACKGROUND: Inflammatory bowel disease (IBD) therapies are modestly successful and associated with significant side effects. Thus, the investigation of novel approaches to prevent colitis is important. Probiotic bacteria can produce immunoregulatory metabolites in vitro such as conjugated linoleic acid (CLA), a polyunsaturated fatty acid with potent anti-inflammatory effects. This study aimed to investigate the cellular and molecular mechanisms underlying the anti-inflammatory efficacy of probiotic bacteria using a mouse model of colitis. METHODOLOGY/PRINCIPAL FINDINGS: The immune modulatory mechanisms of VSL#3 probiotic bacteria and CLA were investigated in a mouse model of DSS colitis. Colonic specimens were collected for histopathology, gene expression and flow cytometry analyses. Immune cell subsets in the mesenteric lymph nodes (MLN), spleen, blood and colonic lamina propria cells were phenotypically and functionally characterized. Fecal samples and colonic contents were collected to determine the effect of VSL#3 and CLA on gut microbial diversity and CLA production. CLA and VSL#3 treatment ameliorated colitis and decreased colonic bacterial diversity, a finding that correlated with decreased gut pathology. Colonic CLA concentrations were increased in response to probiotic bacterial treatment, but without systemic distribution in blood. VSL#3 and CLA decreased macrophage accumulation in the MLN of mice with DSS colitis. The loss of PPAR γ in myeloid cells abrogated the protective effect of probiotic bacteria and CLA in mice with DSS colitis. CONCLUSIONS/SIGNIFICANCE: Probiotic bacteria modulate gut microbial diversity and favor local production of CLA in the colon that targets myeloid cell PPAR γ to suppress colitis. Public Library of Science 2012-02-21 /pmc/articles/PMC3283634/ /pubmed/22363592 http://dx.doi.org/10.1371/journal.pone.0031238 Text en Bassaganya-Riera et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bassaganya-Riera, Josep
Viladomiu, Monica
Pedragosa, Mireia
De Simone, Claudio
Carbo, Adria
Shaykhutdinov, Rustem
Jobin, Christian
Arthur, Janelle C.
Corl, Benjamin A.
Vogel, Hans
Storr, Martin
Hontecillas, Raquel
Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title_full Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title_fullStr Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title_full_unstemmed Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title_short Probiotic Bacteria Produce Conjugated Linoleic Acid Locally in the Gut That Targets Macrophage PPAR γ to Suppress Colitis
title_sort probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage ppar γ to suppress colitis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283634/
https://www.ncbi.nlm.nih.gov/pubmed/22363592
http://dx.doi.org/10.1371/journal.pone.0031238
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