Diversity Measures in Environmental Sequences Are Highly Dependent on Alignment Quality—Data from ITS and New LSU Primers Targeting Basidiomycetes

The ribosomal DNA comprised of the ITS1-5.8S-ITS2 regions is widely used as a fungal marker in molecular ecology and systematics but cannot be aligned with confidence across genetically distant taxa. In order to study the diversity of Agaricomycotina in forest soils, we designed primers targeting th...

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Detalles Bibliográficos
Autores principales: Krüger, Dirk, Kapturska, Danuta, Fischer, Christiane, Daniel, Rolf, Wubet, Tesfaye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283731/
https://www.ncbi.nlm.nih.gov/pubmed/22363808
http://dx.doi.org/10.1371/journal.pone.0032139

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283731/
https://www.ncbi.nlm.nih.gov/pubmed/22363808
http://dx.doi.org/10.1371/journal.pone.0032139

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