Amplified insert assembly: an optimized approach to standard assembly of BioBrick(TM) genetic circuits

A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and digestion with DpnI restriction endonu...

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Detalles Bibliográficos
Autores principales: Speer, Michael A, Richard, Tom L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287150/
https://www.ncbi.nlm.nih.gov/pubmed/22176971
http://dx.doi.org/10.1186/1754-1611-5-17

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287150/
https://www.ncbi.nlm.nih.gov/pubmed/22176971
http://dx.doi.org/10.1186/1754-1611-5-17

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