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Amplified insert assembly: an optimized approach to standard assembly of BioBrick(TM) genetic circuits
A modified BioBrick™ assembly method was developed with higher fidelity than current protocols. The method utilizes a PCR reaction with a standard primer set to amplify the inserted part. Background colonies are reduced by a combination of dephosphorylation and digestion with DpnI restriction endonu...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2011
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3287150/ https://www.ncbi.nlm.nih.gov/pubmed/22176971 http://dx.doi.org/10.1186/1754-1611-5-17 |