A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones

Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...

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Detalles Bibliográficos
Autores principales: Mata, João F., Lopes, Telma, Gardner, Rui, Jansen, Lars E. T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290580/
https://www.ncbi.nlm.nih.gov/pubmed/22393430
http://dx.doi.org/10.1371/journal.pone.0032646

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290580/
https://www.ncbi.nlm.nih.gov/pubmed/22393430
http://dx.doi.org/10.1371/journal.pone.0032646

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