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A Rapid FACS-Based Strategy to Isolate Human Gene Knockin and Knockout Clones
Gene targeting protocols for mammalian cells remain inefficient and labor intensive. Here we describe FASTarget, a rapid, fluorescent cell sorting based strategy to isolate rare gene targeting events in human somatic cells. A fluorescent protein is used as a means for direct selection of targeted cl...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3290580/ https://www.ncbi.nlm.nih.gov/pubmed/22393430 http://dx.doi.org/10.1371/journal.pone.0032646 |