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Agrobacterium tumefaciens-mediated transformation of Aspergillus aculeatus for insertional mutagenesis

Agrobacterium tumefaciens-mediated transformation (AMT) was applied to Aspergillus aculeatus. Transformants carrying the T-DNA from a binary vector pBIG2RHPH2 were sufficiently mitotically stable to allow functional genomic analyses. The AMT technique was optimized by altering the concentration of a...

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Detalles Bibliográficos
Autores principales: Kunitake, Emi, Tani, Shuji, Sumitani, Jun-ichi, Kawaguchi, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292464/
https://www.ncbi.nlm.nih.gov/pubmed/22166586
http://dx.doi.org/10.1186/2191-0855-1-46