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Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase
Legumes and the polyphenolic compounds present in them have gained a lot of interest due to their beneficial health implications. Dietary polyphenolic compounds, especially flavonoids, exert antioxidant properties and are potent inhibitors of xanthine oxidase (XO) activity. XO is the main contributo...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292553/ https://www.ncbi.nlm.nih.gov/pubmed/22396752 http://dx.doi.org/10.1371/journal.pone.0032214 |
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author | Spanou, Chrysoula Veskoukis, Aristidis S. Kerasioti, Thalia Kontou, Maria Angelis, Apostolos Aligiannis, Nektarios Skaltsounis, Alexios-Leandros Kouretas, Dimitrios |
author_facet | Spanou, Chrysoula Veskoukis, Aristidis S. Kerasioti, Thalia Kontou, Maria Angelis, Apostolos Aligiannis, Nektarios Skaltsounis, Alexios-Leandros Kouretas, Dimitrios |
author_sort | Spanou, Chrysoula |
collection | PubMed |
description | Legumes and the polyphenolic compounds present in them have gained a lot of interest due to their beneficial health implications. Dietary polyphenolic compounds, especially flavonoids, exert antioxidant properties and are potent inhibitors of xanthine oxidase (XO) activity. XO is the main contributor of free radicals during exercise but it is also involved in pathogenesis of several diseases such as vascular disorders, cancer and gout. In order to discover new natural, dietary XO inhibitors, some polyphenolic fractions and pure compounds isolated from two legume plant extracts were tested for their effects on XO activity. The fractions isolated from both Vicia faba and Lotus edulis plant extracts were potent inhibitors of XO with IC(50) values range from 40–135 µg/mL and 55–260 µg/mL, respectively. All the pure polyphenolic compounds inhibited XO and their K(i) values ranged from 13–767 µM. Ten of the compounds followed the non competitive inhibitory model whereas one of them was a competitive inhibitor. These findings indicate that flavonoid isolates from legume plant extracts are novel, natural XO inhibitors. Their mode of action is under investigation in order to examine their potential in drug design for diseases related to overwhelming XO action. |
format | Online Article Text |
id | pubmed-3292553 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-32925532012-03-06 Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase Spanou, Chrysoula Veskoukis, Aristidis S. Kerasioti, Thalia Kontou, Maria Angelis, Apostolos Aligiannis, Nektarios Skaltsounis, Alexios-Leandros Kouretas, Dimitrios PLoS One Research Article Legumes and the polyphenolic compounds present in them have gained a lot of interest due to their beneficial health implications. Dietary polyphenolic compounds, especially flavonoids, exert antioxidant properties and are potent inhibitors of xanthine oxidase (XO) activity. XO is the main contributor of free radicals during exercise but it is also involved in pathogenesis of several diseases such as vascular disorders, cancer and gout. In order to discover new natural, dietary XO inhibitors, some polyphenolic fractions and pure compounds isolated from two legume plant extracts were tested for their effects on XO activity. The fractions isolated from both Vicia faba and Lotus edulis plant extracts were potent inhibitors of XO with IC(50) values range from 40–135 µg/mL and 55–260 µg/mL, respectively. All the pure polyphenolic compounds inhibited XO and their K(i) values ranged from 13–767 µM. Ten of the compounds followed the non competitive inhibitory model whereas one of them was a competitive inhibitor. These findings indicate that flavonoid isolates from legume plant extracts are novel, natural XO inhibitors. Their mode of action is under investigation in order to examine their potential in drug design for diseases related to overwhelming XO action. Public Library of Science 2012-03-02 /pmc/articles/PMC3292553/ /pubmed/22396752 http://dx.doi.org/10.1371/journal.pone.0032214 Text en Spanou et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Spanou, Chrysoula Veskoukis, Aristidis S. Kerasioti, Thalia Kontou, Maria Angelis, Apostolos Aligiannis, Nektarios Skaltsounis, Alexios-Leandros Kouretas, Dimitrios Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title | Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title_full | Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title_fullStr | Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title_full_unstemmed | Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title_short | Flavonoid Glycosides Isolated from Unique Legume Plant Extracts as Novel Inhibitors of Xanthine Oxidase |
title_sort | flavonoid glycosides isolated from unique legume plant extracts as novel inhibitors of xanthine oxidase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292553/ https://www.ncbi.nlm.nih.gov/pubmed/22396752 http://dx.doi.org/10.1371/journal.pone.0032214 |
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