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Efficient in vitro RNA interference and immunofluorescence-based phenotype analysis in a human parasitic nematode, Brugia malayi

BACKGROUND: RNA interference (RNAi) is an efficient reverse genetics technique for investigating gene function in eukaryotes. The method has been widely used in model organisms, such as the free-living nematode Caenorhabditis elegans, where it has been deployed in genome-wide high throughput screens...

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Detalles Bibliográficos
Autores principales: Landmann, Frédéric, Foster, Jeremy M, Slatko, Barton E, Sullivan, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3292814/
https://www.ncbi.nlm.nih.gov/pubmed/22243803
http://dx.doi.org/10.1186/1756-3305-5-16