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A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli
BACKGROUND: For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve the problem, for example, the employment of the multi-copy plasmid-based expres...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293061/ https://www.ncbi.nlm.nih.gov/pubmed/22305426 http://dx.doi.org/10.1186/1475-2859-11-19 |