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A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli

BACKGROUND: For metabolic engineering, many rate-limiting steps may exist in the pathways of accumulating the target metabolites. Increasing copy number of the desired genes in these pathways is a general method to solve the problem, for example, the employment of the multi-copy plasmid-based expres...

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Detalles Bibliográficos
Autores principales: Li, Mingji, Wang, Junshu, Geng, Yanping, Li, Yikui, Wang, Qian, Liang, Quanfeng, Qi, Qingsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293061/
https://www.ncbi.nlm.nih.gov/pubmed/22305426
http://dx.doi.org/10.1186/1475-2859-11-19