Selection of recombinant MVA by rescue of the essential D4R gene

Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant...

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Autores principales: Ricci, Patricia S, Schäfer, Birgit, Kreil, Thomas R, Falkner, Falko G, Holzer, Georg W
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2011
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293099/
https://www.ncbi.nlm.nih.gov/pubmed/22152060
http://dx.doi.org/10.1186/1743-422X-8-529
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author Ricci, Patricia S
Schäfer, Birgit
Kreil, Thomas R
Falkner, Falko G
Holzer, Georg W
author_facet Ricci, Patricia S
Schäfer, Birgit
Kreil, Thomas R
Falkner, Falko G
Holzer, Georg W
author_sort Ricci, Patricia S
collection PubMed
description Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.
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spelling pubmed-32930992012-03-05 Selection of recombinant MVA by rescue of the essential D4R gene Ricci, Patricia S Schäfer, Birgit Kreil, Thomas R Falkner, Falko G Holzer, Georg W Virol J Methodology Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant. BioMed Central 2011-12-12 /pmc/articles/PMC3293099/ /pubmed/22152060 http://dx.doi.org/10.1186/1743-422X-8-529 Text en Copyright ©2011 Ricci et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
Ricci, Patricia S
Schäfer, Birgit
Kreil, Thomas R
Falkner, Falko G
Holzer, Georg W
Selection of recombinant MVA by rescue of the essential D4R gene
title Selection of recombinant MVA by rescue of the essential D4R gene
title_full Selection of recombinant MVA by rescue of the essential D4R gene
title_fullStr Selection of recombinant MVA by rescue of the essential D4R gene
title_full_unstemmed Selection of recombinant MVA by rescue of the essential D4R gene
title_short Selection of recombinant MVA by rescue of the essential D4R gene
title_sort selection of recombinant mva by rescue of the essential d4r gene
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293099/
https://www.ncbi.nlm.nih.gov/pubmed/22152060
http://dx.doi.org/10.1186/1743-422X-8-529
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