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Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surfac...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3293749/ https://www.ncbi.nlm.nih.gov/pubmed/22336426 http://dx.doi.org/10.1186/2191-0855-2-12 |