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A cost-effective and universal strategy for complete prokaryotic genomic sequencing proposed by computer simulation
BACKGROUND: Pyrosequencing techniques allow scientists to perform prokaryotic genome sequencing to achieve the draft genomic sequences within a few days. However, the assemblies with shotgun sequencing are usually composed of hundreds of contigs. A further multiplex PCR procedure is needed to fill a...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296665/ https://www.ncbi.nlm.nih.gov/pubmed/22289569 http://dx.doi.org/10.1186/1756-0500-5-80 |