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A cost-effective and universal strategy for complete prokaryotic genomic sequencing proposed by computer simulation

BACKGROUND: Pyrosequencing techniques allow scientists to perform prokaryotic genome sequencing to achieve the draft genomic sequences within a few days. However, the assemblies with shotgun sequencing are usually composed of hundreds of contigs. A further multiplex PCR procedure is needed to fill a...

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Detalles Bibliográficos
Autores principales: Jiang, Jingwei, Li, Jun, Kwan, Hoi Shan, Au, Chun Hang, Wan Law, Patrick Tik, Li, Lei, Kam, Kai Man, Lun Ling, Julia Mei, Leung, Frederick C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296665/
https://www.ncbi.nlm.nih.gov/pubmed/22289569
http://dx.doi.org/10.1186/1756-0500-5-80