Selection of Diethylstilbestrol-Specific Single-Chain Antibodies from a Non-Immunized Mouse Ribosome Display Library

Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Yanan, Ning, Baoan, Liu, Ming, Gao, Xianjun, Fan, Xianjun, Liu, Jianqing, Gao, Zhixian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302827/
https://www.ncbi.nlm.nih.gov/pubmed/22427984
http://dx.doi.org/10.1371/journal.pone.0033186

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302827/
https://www.ncbi.nlm.nih.gov/pubmed/22427984
http://dx.doi.org/10.1371/journal.pone.0033186

Ejemplares similares