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Selection of Diethylstilbestrol-Specific Single-Chain Antibodies from a Non-Immunized Mouse Ribosome Display Library

Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating...

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Detalles Bibliográficos
Autores principales:	Sun, Yanan, Ning, Baoan, Liu, Ming, Gao, Xianjun, Fan, Xianjun, Liu, Jianqing, Gao, Zhixian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302827/ https://www.ncbi.nlm.nih.gov/pubmed/22427984 http://dx.doi.org/10.1371/journal.pone.0033186

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