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Selection of Diethylstilbestrol-Specific Single-Chain Antibodies from a Non-Immunized Mouse Ribosome Display Library
Single chain variable fragments (scFvs) against diethylstilbestrol (DES) were selected from the splenocytes of non-immunized mice by ribosome display technology. A naive library was constructed and engineered to allow in vitro transcription and translation using an E. coli lysate system. Alternating...
Autores principales: | Sun, Yanan, Ning, Baoan, Liu, Ming, Gao, Xianjun, Fan, Xianjun, Liu, Jianqing, Gao, Zhixian |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302827/ https://www.ncbi.nlm.nih.gov/pubmed/22427984 http://dx.doi.org/10.1371/journal.pone.0033186 |
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