Cargando…

Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate

We present direct evidence of an activator-inhibitor system in the generation of the regularly spaced transverse ridges of the palate. We show that new ridges, or rugae, marked by stripes of Sonic hedgehog (Shh) expression, appear at two growth zones where the space between previously laid-down ruga...

Descripción completa

Detalles Bibliográficos
Autores principales: Economou, Andrew D., Ohazama, Atsushi, Porntaveetus, Thantrira, Sharpe, Paul T., Kondo, Shigeru, Basson, M. Albert, Gritli-Linde, Amel, Cobourne, Martyn T., Green, Jeremy B.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303118/
https://www.ncbi.nlm.nih.gov/pubmed/22344222
http://dx.doi.org/10.1038/ng.1090
_version_ 1782226722634072064
author Economou, Andrew D.
Ohazama, Atsushi
Porntaveetus, Thantrira
Sharpe, Paul T.
Kondo, Shigeru
Basson, M. Albert
Gritli-Linde, Amel
Cobourne, Martyn T.
Green, Jeremy B.A.
author_facet Economou, Andrew D.
Ohazama, Atsushi
Porntaveetus, Thantrira
Sharpe, Paul T.
Kondo, Shigeru
Basson, M. Albert
Gritli-Linde, Amel
Cobourne, Martyn T.
Green, Jeremy B.A.
author_sort Economou, Andrew D.
collection PubMed
description We present direct evidence of an activator-inhibitor system in the generation of the regularly spaced transverse ridges of the palate. We show that new ridges, or rugae, marked by stripes of Sonic hedgehog (Shh) expression, appear at two growth zones where the space between previously laid-down rugae increases. However, inter-rugal growth is not absolutely required: new stripes still appear when growth is inhibited. Furthermore, when a ruga is excised new Shh expression appears, not at the cut edge but as bifurcating stripes branching from the neighbouring Shh stripe, diagnostic of a Turing-type reaction-diffusion mechanism. Genetic and inhibitor experiments identify Fibroblast Growth Factor (FGF) and Shh as an activator-inhibitor pair in this system. These findings demonstrate a reaction-diffusion mechanism likely to be widely relevant in vertebrate development.
format Online
Article
Text
id pubmed-3303118
institution National Center for Biotechnology Information
language English
publishDate 2012
record_format MEDLINE/PubMed
spelling pubmed-33031182012-09-01 Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate Economou, Andrew D. Ohazama, Atsushi Porntaveetus, Thantrira Sharpe, Paul T. Kondo, Shigeru Basson, M. Albert Gritli-Linde, Amel Cobourne, Martyn T. Green, Jeremy B.A. Nat Genet Article We present direct evidence of an activator-inhibitor system in the generation of the regularly spaced transverse ridges of the palate. We show that new ridges, or rugae, marked by stripes of Sonic hedgehog (Shh) expression, appear at two growth zones where the space between previously laid-down rugae increases. However, inter-rugal growth is not absolutely required: new stripes still appear when growth is inhibited. Furthermore, when a ruga is excised new Shh expression appears, not at the cut edge but as bifurcating stripes branching from the neighbouring Shh stripe, diagnostic of a Turing-type reaction-diffusion mechanism. Genetic and inhibitor experiments identify Fibroblast Growth Factor (FGF) and Shh as an activator-inhibitor pair in this system. These findings demonstrate a reaction-diffusion mechanism likely to be widely relevant in vertebrate development. 2012-02-19 /pmc/articles/PMC3303118/ /pubmed/22344222 http://dx.doi.org/10.1038/ng.1090 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Economou, Andrew D.
Ohazama, Atsushi
Porntaveetus, Thantrira
Sharpe, Paul T.
Kondo, Shigeru
Basson, M. Albert
Gritli-Linde, Amel
Cobourne, Martyn T.
Green, Jeremy B.A.
Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title_full Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title_fullStr Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title_full_unstemmed Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title_short Periodic stripe formation by a Turing-mechanism operating at growth zones in the mammalian palate
title_sort periodic stripe formation by a turing-mechanism operating at growth zones in the mammalian palate
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3303118/
https://www.ncbi.nlm.nih.gov/pubmed/22344222
http://dx.doi.org/10.1038/ng.1090
work_keys_str_mv AT economouandrewd periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT ohazamaatsushi periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT porntaveetusthantrira periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT sharpepault periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT kondoshigeru periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT bassonmalbert periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT gritlilindeamel periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT cobournemartynt periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate
AT greenjeremyba periodicstripeformationbyaturingmechanismoperatingatgrowthzonesinthemammalianpalate