Expression and localization of claudins-3 and -12 in transformed human brain endothelium

BACKGROUND: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. FINDINGS: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ...

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Autores principales: Schrade, Anja, Sade, Hadassah, Couraud, Pierre-Olivier, Romero, Ignacio A, Weksler, Babette B, Niewoehner, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Short Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305566/
https://www.ncbi.nlm.nih.gov/pubmed/22373538
http://dx.doi.org/10.1186/2045-8118-9-6
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author Schrade, Anja
Sade, Hadassah
Couraud, Pierre-Olivier
Romero, Ignacio A
Weksler, Babette B
Niewoehner, Jens
author_facet Schrade, Anja
Sade, Hadassah
Couraud, Pierre-Olivier
Romero, Ignacio A
Weksler, Babette B
Niewoehner, Jens
author_sort Schrade, Anja
collection PubMed
description BACKGROUND: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. FINDINGS: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. CONCLUSIONS: These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
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spelling pubmed-33055662012-03-16 Expression and localization of claudins-3 and -12 in transformed human brain endothelium Schrade, Anja Sade, Hadassah Couraud, Pierre-Olivier Romero, Ignacio A Weksler, Babette B Niewoehner, Jens Fluids Barriers CNS Short Paper BACKGROUND: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. FINDINGS: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. CONCLUSIONS: These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro. BioMed Central 2012-02-29 /pmc/articles/PMC3305566/ /pubmed/22373538 http://dx.doi.org/10.1186/2045-8118-9-6 Text en Copyright ©2012 Schrade et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Paper
Schrade, Anja
Sade, Hadassah
Couraud, Pierre-Olivier
Romero, Ignacio A
Weksler, Babette B
Niewoehner, Jens
Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title_full Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title_fullStr Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title_full_unstemmed Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title_short Expression and localization of claudins-3 and -12 in transformed human brain endothelium
title_sort expression and localization of claudins-3 and -12 in transformed human brain endothelium
topic Short Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3305566/
https://www.ncbi.nlm.nih.gov/pubmed/22373538
http://dx.doi.org/10.1186/2045-8118-9-6
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