A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dep...

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Detalles Bibliográficos
Autores principales: Roux, Kyle J., Kim, Dae In, Raida, Manfred, Burke, Brian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Tools
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308701/
https://www.ncbi.nlm.nih.gov/pubmed/22412018
http://dx.doi.org/10.1083/jcb.201112098

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308701/
https://www.ncbi.nlm.nih.gov/pubmed/22412018
http://dx.doi.org/10.1083/jcb.201112098

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