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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells
We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dep...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3308701/ https://www.ncbi.nlm.nih.gov/pubmed/22412018 http://dx.doi.org/10.1083/jcb.201112098 |