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Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat

A fluorescent voltage sensor protein “Flare” was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular region of visual cortex in rat. Injection sites were selected based...

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Autores principales: Ahrens, Kurt F., Heider, Barbara, Lee, Hanson, Isacoff, Ehud Y., Siegel, Ralph M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3310150/
https://www.ncbi.nlm.nih.gov/pubmed/22461770
http://dx.doi.org/10.3389/fncir.2012.00015
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author Ahrens, Kurt F.
Heider, Barbara
Lee, Hanson
Isacoff, Ehud Y.
Siegel, Ralph M.
author_facet Ahrens, Kurt F.
Heider, Barbara
Lee, Hanson
Isacoff, Ehud Y.
Siegel, Ralph M.
author_sort Ahrens, Kurt F.
collection PubMed
description A fluorescent voltage sensor protein “Flare” was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular region of visual cortex in rat. Injection sites were selected based on intrinsic optical imaging. Expression of Flare occurred in the cell bodies and dendritic processes. Neurons imaged in vivo using two-photon scanning microscopy typically revealed the soma best, discernable against the background labeling of the neuropil. Somatic fluorescence changes were correlated with flashed visual stimuli; however, averaging was essential to observe these changes. This study demonstrates that the genetic modification of single neurons to express a fluorescent voltage sensor can be used to assess neuronal activity in vivo.
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spelling pubmed-33101502012-03-29 Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat Ahrens, Kurt F. Heider, Barbara Lee, Hanson Isacoff, Ehud Y. Siegel, Ralph M. Front Neural Circuits Neuroscience A fluorescent voltage sensor protein “Flare” was created from a Kv1.4 potassium channel with YFP situated to report voltage-induced conformational changes in vivo. The RNA virus Sindbis introduced Flare into neurons in the binocular region of visual cortex in rat. Injection sites were selected based on intrinsic optical imaging. Expression of Flare occurred in the cell bodies and dendritic processes. Neurons imaged in vivo using two-photon scanning microscopy typically revealed the soma best, discernable against the background labeling of the neuropil. Somatic fluorescence changes were correlated with flashed visual stimuli; however, averaging was essential to observe these changes. This study demonstrates that the genetic modification of single neurons to express a fluorescent voltage sensor can be used to assess neuronal activity in vivo. Frontiers Media S.A. 2012-03-22 /pmc/articles/PMC3310150/ /pubmed/22461770 http://dx.doi.org/10.3389/fncir.2012.00015 Text en Copyright © 2012 Ahrens, Heider, Lee, Isacoff and Siegel. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.
spellingShingle Neuroscience
Ahrens, Kurt F.
Heider, Barbara
Lee, Hanson
Isacoff, Ehud Y.
Siegel, Ralph M.
Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title_full Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title_fullStr Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title_full_unstemmed Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title_short Two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
title_sort two-photon scanning microscopy of in vivo sensory responses of cortical neurons genetically encoded with a fluorescent voltage sensor in rat
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3310150/
https://www.ncbi.nlm.nih.gov/pubmed/22461770
http://dx.doi.org/10.3389/fncir.2012.00015
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