Improved Detection of Bifidobacteria with Optimised 16S rRNA-Gene Based Pyrosequencing

The 16S rRNA gene is conserved across all bacteria and as such is routinely targeted in PCR surveys of bacterial diversity. PCR primer design aims to amplify as many different 16S rRNA gene sequences from as wide a range of organisms as possible, though there are no suitable 100% conserved regions o...

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Detalles Bibliográficos
Autores principales: Sim, Kathleen, Cox, Michael J., Wopereis, Harm, Martin, Rocio, Knol, Jan, Li, Ming-Shi, Cookson, William O. C. M., Moffatt, Miriam F., Kroll, J. Simon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://www.ncbi.nlm.nih.gov/pubmed/22470420
http://dx.doi.org/10.1371/journal.pone.0032543

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3314643/
https://www.ncbi.nlm.nih.gov/pubmed/22470420
http://dx.doi.org/10.1371/journal.pone.0032543

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