High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins

Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance ass...

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Detalles Bibliográficos
Autores principales: Zawadzka-Kazimierczuk, Anna, Koźmiński, Wiktor, Šanderová, Hana, Krásný, Libor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315646/
https://www.ncbi.nlm.nih.gov/pubmed/22350953
http://dx.doi.org/10.1007/s10858-012-9613-x
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author Zawadzka-Kazimierczuk, Anna
Koźmiński, Wiktor
Šanderová, Hana
Krásný, Libor
author_facet Zawadzka-Kazimierczuk, Anna
Koźmiński, Wiktor
Šanderová, Hana
Krásný, Libor
author_sort Zawadzka-Kazimierczuk, Anna
collection PubMed
description Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences.
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spelling pubmed-33156462012-04-05 High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins Zawadzka-Kazimierczuk, Anna Koźmiński, Wiktor Šanderová, Hana Krásný, Libor J Biomol NMR Article Four novel 5D (HACA(N)CONH, HNCOCACB, (HACA)CON(CA)CONH, (H)NCO(NCA)CONH), and one 6D ((H)NCO(N)CACONH) NMR pulse sequences are proposed. The new experiments employ non-uniform sampling that enables achieving high resolution in indirectly detected dimensions. The experiments facilitate resonance assignment of intrinsically disordered proteins. The novel pulse sequences were successfully tested using δ subunit (20 kDa) of Bacillus subtilis RNA polymerase that has an 81-amino acid disordered part containing various repetitive sequences. Springer Netherlands 2012-02-17 2012 /pmc/articles/PMC3315646/ /pubmed/22350953 http://dx.doi.org/10.1007/s10858-012-9613-x Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Zawadzka-Kazimierczuk, Anna
Koźmiński, Wiktor
Šanderová, Hana
Krásný, Libor
High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title_full High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title_fullStr High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title_full_unstemmed High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title_short High dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
title_sort high dimensional and high resolution pulse sequences for backbone resonance assignment of intrinsically disordered proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315646/
https://www.ncbi.nlm.nih.gov/pubmed/22350953
http://dx.doi.org/10.1007/s10858-012-9613-x
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