Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors

Histone deacetylase inhibitors (HDACi) are increasingly used as therapeutic agents, but the mechanisms by which they alter cell behaviour remain unclear. Here we use microarray expression analysis to show that only a small proportion of genes (∼9%) have altered transcript levels after treating HL60...

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Autores principales: Halsall, John, Gupta, Vibhor, O'Neill, Laura P., Turner, Bryan M., Nightingale, Karl P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316569/
https://www.ncbi.nlm.nih.gov/pubmed/22479401
http://dx.doi.org/10.1371/journal.pone.0033453
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author Halsall, John
Gupta, Vibhor
O'Neill, Laura P.
Turner, Bryan M.
Nightingale, Karl P.
author_facet Halsall, John
Gupta, Vibhor
O'Neill, Laura P.
Turner, Bryan M.
Nightingale, Karl P.
author_sort Halsall, John
collection PubMed
description Histone deacetylase inhibitors (HDACi) are increasingly used as therapeutic agents, but the mechanisms by which they alter cell behaviour remain unclear. Here we use microarray expression analysis to show that only a small proportion of genes (∼9%) have altered transcript levels after treating HL60 cells with different HDACi (valproic acid, Trichostatin A, suberoylanilide hydroxamic acid). Different gene populations respond to each inhibitor, with as many genes down- as up-regulated. Surprisingly, HDACi rarely induced increased histone acetylation at gene promoters, with most genes examined showing minimal change, irrespective of whether genes were up- or down-regulated. Many genes seem to be sheltered from the global histone hyperacetyation induced by HDACi.
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spelling pubmed-33165692012-04-04 Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors Halsall, John Gupta, Vibhor O'Neill, Laura P. Turner, Bryan M. Nightingale, Karl P. PLoS One Research Article Histone deacetylase inhibitors (HDACi) are increasingly used as therapeutic agents, but the mechanisms by which they alter cell behaviour remain unclear. Here we use microarray expression analysis to show that only a small proportion of genes (∼9%) have altered transcript levels after treating HL60 cells with different HDACi (valproic acid, Trichostatin A, suberoylanilide hydroxamic acid). Different gene populations respond to each inhibitor, with as many genes down- as up-regulated. Surprisingly, HDACi rarely induced increased histone acetylation at gene promoters, with most genes examined showing minimal change, irrespective of whether genes were up- or down-regulated. Many genes seem to be sheltered from the global histone hyperacetyation induced by HDACi. Public Library of Science 2012-03-30 /pmc/articles/PMC3316569/ /pubmed/22479401 http://dx.doi.org/10.1371/journal.pone.0033453 Text en Halsall et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Halsall, John
Gupta, Vibhor
O'Neill, Laura P.
Turner, Bryan M.
Nightingale, Karl P.
Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title_full Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title_fullStr Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title_full_unstemmed Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title_short Genes Are Often Sheltered from the Global Histone Hyperacetylation Induced by HDAC Inhibitors
title_sort genes are often sheltered from the global histone hyperacetylation induced by hdac inhibitors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316569/
https://www.ncbi.nlm.nih.gov/pubmed/22479401
http://dx.doi.org/10.1371/journal.pone.0033453
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