A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines

Mass spectrometry-based proteomics increasingly relies on relative or absolute quantification. In relative quantification, stable isotope based methods often allow mixing at early stages of sample preparation, whereas for absolute quantification this has generally required recombinant expression of...

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Detalles Bibliográficos
Autores principales: Zeiler, Marlis, Straube, Werner L., Lundberg, Emma, Uhlen, Mathias, Mann, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Biochemistry and Molecular Biology 2012
Materias:
Special Issue: Prospects in Space and Time
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316735/
https://www.ncbi.nlm.nih.gov/pubmed/21964433
http://dx.doi.org/10.1074/mcp.O111.009613

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3316735/
https://www.ncbi.nlm.nih.gov/pubmed/21964433
http://dx.doi.org/10.1074/mcp.O111.009613

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