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Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine
Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX te...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317715/ https://www.ncbi.nlm.nih.gov/pubmed/22489154 http://dx.doi.org/10.3390/ijms13033341 |
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author | Ye, Mao Hu, Jun Peng, Minyuan Liu, Jing Liu, Jun Liu, Huixia Zhao, Xielan Tan, Weihong |
author_facet | Ye, Mao Hu, Jun Peng, Minyuan Liu, Jing Liu, Jun Liu, Huixia Zhao, Xielan Tan, Weihong |
author_sort | Ye, Mao |
collection | PubMed |
description | Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery. |
format | Online Article Text |
id | pubmed-3317715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-33177152012-04-09 Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine Ye, Mao Hu, Jun Peng, Minyuan Liu, Jing Liu, Jun Liu, Huixia Zhao, Xielan Tan, Weihong Int J Mol Sci Review Aptamers are single-stranded oligonucleotides of DNA or RNA that bind to target molecules with high affinity and specificity. Typically, aptamers are generated by an iterative selection process, called systematic evolution of ligands by exponential enrichment (SELEX). Recent advancements in SELEX technology have extended aptamer selection from comparatively simple mixtures of purified proteins to whole living cells, and now cell-based SELEX (or cell-SELEX) can isolate aptamers that bind to specific target cells. Combined with nanotechnology, microchips, microfluidic devices, RNAi and other advanced technologies, cell-SELEX represents an integrated platform providing ultrasensitive and highly specific tools for clinical medicine. In this review, we describe the recent progress made in the application of cell-SELEX for diagnosis, therapy and biomarker discovery. Molecular Diversity Preservation International (MDPI) 2012-03-12 /pmc/articles/PMC3317715/ /pubmed/22489154 http://dx.doi.org/10.3390/ijms13033341 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Ye, Mao Hu, Jun Peng, Minyuan Liu, Jing Liu, Jun Liu, Huixia Zhao, Xielan Tan, Weihong Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title | Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title_full | Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title_fullStr | Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title_full_unstemmed | Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title_short | Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine |
title_sort | generating aptamers by cell-selex for applications in molecular medicine |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317715/ https://www.ncbi.nlm.nih.gov/pubmed/22489154 http://dx.doi.org/10.3390/ijms13033341 |
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