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Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy
The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), of which ZP2 is proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage is associated with exocytosis of cortical granules that are peripherally located subcellular organelles unique to ovulated...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317803/ https://www.ncbi.nlm.nih.gov/pubmed/22472438 http://dx.doi.org/10.1083/jcb.201112094 |
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author | Burkart, Anna D. Xiong, Bo Baibakov, Boris Jiménez-Movilla, Maria Dean, Jurrien |
author_facet | Burkart, Anna D. Xiong, Bo Baibakov, Boris Jiménez-Movilla, Maria Dean, Jurrien |
author_sort | Burkart, Anna D. |
collection | PubMed |
description | The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), of which ZP2 is proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage is associated with exocytosis of cortical granules that are peripherally located subcellular organelles unique to ovulated eggs. Based on the cleavage site of ZP2, ovastacin was selected as a candidate protease. Encoded by the single-copy Astl gene, ovastacin is an oocyte-specific member of the astacin family of metalloendoproteases. Using specific antiserum, ovastacin was detected in cortical granules before, but not after, fertilization. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease. Female mice lacking ovastacin did not cleave ZP2 after fertilization, and mouse sperm bound as well to Astl-null two-cell embryos as they did to normal eggs. Ovastacin is a pioneer component of mouse cortical granules and plays a definitive role in the postfertilization block to sperm binding that ensures monospermic fertilization and successful development. |
format | Online Article Text |
id | pubmed-3317803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33178032012-10-02 Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy Burkart, Anna D. Xiong, Bo Baibakov, Boris Jiménez-Movilla, Maria Dean, Jurrien J Cell Biol Research Articles The mouse zona pellucida is composed of three glycoproteins (ZP1, ZP2, and ZP3), of which ZP2 is proteolytically cleaved after gamete fusion to prevent polyspermy. This cleavage is associated with exocytosis of cortical granules that are peripherally located subcellular organelles unique to ovulated eggs. Based on the cleavage site of ZP2, ovastacin was selected as a candidate protease. Encoded by the single-copy Astl gene, ovastacin is an oocyte-specific member of the astacin family of metalloendoproteases. Using specific antiserum, ovastacin was detected in cortical granules before, but not after, fertilization. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease. Female mice lacking ovastacin did not cleave ZP2 after fertilization, and mouse sperm bound as well to Astl-null two-cell embryos as they did to normal eggs. Ovastacin is a pioneer component of mouse cortical granules and plays a definitive role in the postfertilization block to sperm binding that ensures monospermic fertilization and successful development. The Rockefeller University Press 2012-04-02 /pmc/articles/PMC3317803/ /pubmed/22472438 http://dx.doi.org/10.1083/jcb.201112094 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Burkart, Anna D. Xiong, Bo Baibakov, Boris Jiménez-Movilla, Maria Dean, Jurrien Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title | Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title_full | Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title_fullStr | Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title_full_unstemmed | Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title_short | Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy |
title_sort | ovastacin, a cortical granule protease, cleaves zp2 in the zona pellucida to prevent polyspermy |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317803/ https://www.ncbi.nlm.nih.gov/pubmed/22472438 http://dx.doi.org/10.1083/jcb.201112094 |
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