Small, N-Terminal Tags Activate Parkin E3 Ubiquitin Ligase Activity by Disrupting Its Autoinhibited Conformation

Parkin is an E3 ubiquitin ligase, mutations in which cause Autosomal Recessive Parkinson's Disease. Many studies aimed at understanding Parkin function, regulation and dysfunction are performed using N-terminal epitope tags. We report here that the use of small tags such as FLAG, cMyc and HA, i...

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Detalles Bibliográficos
Autores principales: Burchell, Lynn, Chaugule, Viduth K., Walden, Helen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3319606/
https://www.ncbi.nlm.nih.gov/pubmed/22496854
http://dx.doi.org/10.1371/journal.pone.0034748

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3319606/
https://www.ncbi.nlm.nih.gov/pubmed/22496854
http://dx.doi.org/10.1371/journal.pone.0034748

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