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Performance Analysis of Orthogonal Pairs Designed for an Expanded Eukaryotic Genetic Code

BACKGROUND: The suppression of amber stop codons with non-canonical amino acids (ncAAs) is used for the site-specific introduction of many unusual functions into proteins. Specific orthogonal aminoacyl-tRNA synthetase (o-aaRS)/amber suppressor tRNA(CUA) pairs (o-pairs) for the incorporation of ncAAs...

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Detalles Bibliográficos
Autores principales: Nehring, Sebastian, Budisa, Nediljko, Wiltschi, Birgit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320878/
https://www.ncbi.nlm.nih.gov/pubmed/22493661
http://dx.doi.org/10.1371/journal.pone.0031992