A Rapid, Cost-Effective Method of Assembly and Purification of Synthetic DNA Probes >100 bp

Here we introduce a rapid, cost-effective method of generating molecular DNA probes in just under 15 minutes without the need for expensive, time-consuming gel-extraction steps. As an example, we enzymatically concatenated six variable strands (50 bp) with a common strand sequence (51 bp) in a singl...

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Detalles Bibliográficos
Autores principales: Jensen, Michael A., Jauregui, Lauren, Davis, Ronald W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321010/
https://www.ncbi.nlm.nih.gov/pubmed/22493688
http://dx.doi.org/10.1371/journal.pone.0034373

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321010/
https://www.ncbi.nlm.nih.gov/pubmed/22493688
http://dx.doi.org/10.1371/journal.pone.0034373

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