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A Rapid, Cost-Effective Method of Assembly and Purification of Synthetic DNA Probes >100 bp
Here we introduce a rapid, cost-effective method of generating molecular DNA probes in just under 15 minutes without the need for expensive, time-consuming gel-extraction steps. As an example, we enzymatically concatenated six variable strands (50 bp) with a common strand sequence (51 bp) in a singl...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2012
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321010/ https://www.ncbi.nlm.nih.gov/pubmed/22493688 http://dx.doi.org/10.1371/journal.pone.0034373 |