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A GFP-lacZ Bicistronic Reporter System for Promoter Analysis in Environmental Gram-Negative Bacteria

Here, we describe a bicistronic reporter system for the analysis of promoter activity in a variety of Gram-negative bacteria at both the population and single-cell levels. This synthetic genetic tool utilizes an artificial operon comprising the gfp and lacZ genes that are assembled in a suicide vect...

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Detalles Bibliográficos
Autores principales: Silva-Rocha, Rafael, de Lorenzo, Victor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321037/
https://www.ncbi.nlm.nih.gov/pubmed/22493710
http://dx.doi.org/10.1371/journal.pone.0034675