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Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies
HIV-1's subtype C V3 loop consensus sequence exhibits increased resistance to anti-V3 antibody-mediated neutralization as compared to the subtype B consensus sequence. The dynamic 3D structure of the consensus C V3 loop crown, visualized by ab initio folding, suggested that the resistance deriv...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323838/ https://www.ncbi.nlm.nih.gov/pubmed/22548061 http://dx.doi.org/10.1155/2012/803535 |
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author | Almond, David Krachmarov, Chavdar Swetnam, James Zolla-Pazner, Susan Cardozo, Timothy |
author_facet | Almond, David Krachmarov, Chavdar Swetnam, James Zolla-Pazner, Susan Cardozo, Timothy |
author_sort | Almond, David |
collection | PubMed |
description | HIV-1's subtype C V3 loop consensus sequence exhibits increased resistance to anti-V3 antibody-mediated neutralization as compared to the subtype B consensus sequence. The dynamic 3D structure of the consensus C V3 loop crown, visualized by ab initio folding, suggested that the resistance derives from structural rigidity and non-β-strand secondary protein structure in the N-terminal strand of the β-hairpin of the V3 loop crown, which is where most known anti-V3 loop antibodies bind. The observation of either rigidity or non-β-strand structure in this region correlated with observed resistance to antibody-mediated neutralization in a series of chimeric pseudovirus (psV) mutants. The results suggest the presence of an epitope-independent, neutralization-relevant structural difference in the antibody-targeted region of the V3 loop crown between subtype C and subtype B, a difference that we hypothesize may contribute to the divergent pattern of global spread between these subtypes. As antibodies to a variable loop were recently identified as an inverse correlate of risk for HIV infection, the structure-function relationships discussed in this study may have relevance to HIV vaccine research. |
format | Online Article Text |
id | pubmed-3323838 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33238382012-04-30 Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies Almond, David Krachmarov, Chavdar Swetnam, James Zolla-Pazner, Susan Cardozo, Timothy Adv Virol Research Article HIV-1's subtype C V3 loop consensus sequence exhibits increased resistance to anti-V3 antibody-mediated neutralization as compared to the subtype B consensus sequence. The dynamic 3D structure of the consensus C V3 loop crown, visualized by ab initio folding, suggested that the resistance derives from structural rigidity and non-β-strand secondary protein structure in the N-terminal strand of the β-hairpin of the V3 loop crown, which is where most known anti-V3 loop antibodies bind. The observation of either rigidity or non-β-strand structure in this region correlated with observed resistance to antibody-mediated neutralization in a series of chimeric pseudovirus (psV) mutants. The results suggest the presence of an epitope-independent, neutralization-relevant structural difference in the antibody-targeted region of the V3 loop crown between subtype C and subtype B, a difference that we hypothesize may contribute to the divergent pattern of global spread between these subtypes. As antibodies to a variable loop were recently identified as an inverse correlate of risk for HIV infection, the structure-function relationships discussed in this study may have relevance to HIV vaccine research. Hindawi Publishing Corporation 2012 2012-04-02 /pmc/articles/PMC3323838/ /pubmed/22548061 http://dx.doi.org/10.1155/2012/803535 Text en Copyright © 2012 David Almond et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Almond, David Krachmarov, Chavdar Swetnam, James Zolla-Pazner, Susan Cardozo, Timothy Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title | Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title_full | Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title_fullStr | Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title_full_unstemmed | Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title_short | Resistance of Subtype C HIV-1 Strains to Anti-V3 Loop Antibodies |
title_sort | resistance of subtype c hiv-1 strains to anti-v3 loop antibodies |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323838/ https://www.ncbi.nlm.nih.gov/pubmed/22548061 http://dx.doi.org/10.1155/2012/803535 |
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