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Revised Selection Criteria for Candidate Restriction Enzymes in Genome Walking

A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps. These criteria include: step (1) initially choosing the most appropriat...

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Detalles Bibliográficos
Autores principales: Taheri, Ali, Robinson, Stephen J., Parkin, Isobel, Gruber, Margaret Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324424/
https://www.ncbi.nlm.nih.gov/pubmed/22509391
http://dx.doi.org/10.1371/journal.pone.0035117