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Comparison of DNA Extraction Methods for Microbial Community Profiling with an Application to Pediatric Bronchoalveolar Lavage Samples

Barcoded amplicon sequencing is rapidly becoming a standard method for profiling microbial communities, including the human respiratory microbiome. While this approach has less bias than standard cultivation, several steps can introduce variation including the type of DNA extraction method used. Her...

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Detalles Bibliográficos
Autores principales: Willner, Dana, Daly, Joshua, Whiley, David, Grimwood, Keith, Wainwright, Claire E., Hugenholtz, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326054/
https://www.ncbi.nlm.nih.gov/pubmed/22514642
http://dx.doi.org/10.1371/journal.pone.0034605