Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime

Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR). Currently, there is no alternative to RT(−) controls to evaluate the impact of the gDNA background on...

Descripción completa

Detalles Bibliográficos
Autores principales: Laurell, Henrik, Iacovoni, Jason S., Abot, Anne, Svec, David, Maoret, Jean-José, Arnal, Jean-François, Kubista, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326333/
https://www.ncbi.nlm.nih.gov/pubmed/22228834
http://dx.doi.org/10.1093/nar/gkr1259

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326333/
https://www.ncbi.nlm.nih.gov/pubmed/22228834
http://dx.doi.org/10.1093/nar/gkr1259

Ejemplares similares