Cargando…

Correction of RT–qPCR data for genomic DNA-derived signals with ValidPrime

Genomic DNA (gDNA) contamination is an inherent problem during RNA purification that can lead to non-specific amplification and aberrant results in reverse transcription quantitative PCR (RT—qPCR). Currently, there is no alternative to RT(−) controls to evaluate the impact of the gDNA background on...

Descripción completa

Detalles Bibliográficos
Autores principales:	Laurell, Henrik, Iacovoni, Jason S., Abot, Anne, Svec, David, Maoret, Jean-José, Arnal, Jean-François, Kubista, Mikael
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3326333/ https://www.ncbi.nlm.nih.gov/pubmed/22228834 http://dx.doi.org/10.1093/nar/gkr1259

Ejemplares similares

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

Tutorial: Guidelines for Single-Cell RT-qPCR
por: Zucha, Daniel, et al.
Publicado: (2021)

Quantification of siRNA using competitive qPCR
por: Liu, Wei-li, et al.
Publicado: (2009)

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)

OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
por: He, Fang, et al.
Publicado: (2021)

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
por: Shaik, Gouse M., et al.
Publicado: (2008)

ΩqPCR measures telomere length from single-cells in base pair units
por: Xiong, Fusheng, et al.
Publicado: (2021)

A novel and universal method for microRNA RT-qPCR data normalization
por: Mestdagh, Pieter, et al.
Publicado: (2009)

Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR
por: Tan, Ke-En, et al.
Publicado: (2023)

How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments
por: Svec, David, et al.
Publicado: (2015)

NormQ: RNASeq normalization based on RT-qPCR derived size factors
por: Naraine, Ravindra, et al.
Publicado: (2020)

Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR
por: Aviv, Abraham, et al.
Publicado: (2011)

Validation of kinetics similarity in qPCR
por: Bar, Tzachi, et al.
Publicado: (2012)

Detection of SARS-CoV-2 RNA by multiplex RT-qPCR
por: Kudo, Eriko, et al.
Publicado: (2020)

An improved RT-qPCR method for direct quantification of enveloped RNA viruses
por: Gregorova, Pavlina, et al.
Publicado: (2022)

Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery
por: Voss, Gjendine, et al.
Publicado: (2021)

Two-tailed RT-qPCR panel for quality control of circulating microRNA studies
por: Androvic, Peter, et al.
Publicado: (2019)

Evidence‐based selection of reference genes for RT‐qPCR assays in periodontal research
por: Diehl, Daniel, et al.
Publicado: (2022)

Evaluation of critical design parameters for RT‐qPCR‐based analysis of multiple dUTPase isoform genes in mice
por: Rácz, Gergely A., et al.
Publicado: (2019)

A sensitive and affordable multiplex RT-qPCR assay for SARS-CoV-2 detection
por: Reijns, Martin A. M., et al.
Publicado: (2020)

Miniature RT–PCR system for diagnosis of RNA-based viruses
por: Liao, Chia-Sheng, et al.
Publicado: (2005)

Real-time quantification of microRNAs by stem–loop RT–PCR
por: Chen, Caifu, et al.
Publicado: (2005)

Design, development, and validation of a strand-specific RT-qPCR assay for GI and GII human Noroviruses
por: König, Katja Marie Kjara, et al.
Publicado: (2021)

Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi
por: Llanos, Agustina, et al.
Publicado: (2015)

Expression Stability Analysis of Candidate References for Normalization of RT-qPCR Data Using RefSeeker R package
por: Petersen, Patrick H.D., et al.
Publicado: (2023)

Methods to determine limit of detection and limit of quantification in quantitative real-time PCR (qPCR)
por: Forootan, Amin, et al.
Publicado: (2017)

qKAT: a high-throughput qPCR method for KIR gene copy number and haplotype determination
por: Jiang, W., et al.
Publicado: (2016)

Telomere length measurement by qPCR in birds is affected by storage method of blood samples
por: Reichert, Sophie, et al.
Publicado: (2017)

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR
por: Fout, G. Shay, et al.
Publicado: (2016)

Identification of Reference Genes for RT-qPCR Data Normalization in Cannabis sativa Stem Tissues
por: Mangeot-Peter, Lauralie, et al.
Publicado: (2016)

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol
por: Chovancova, Petra, et al.
Publicado: (2017)

Direct RT-qPCR detection of SARS-CoV-2 RNA from patient nasopharyngeal swabs without an RNA extraction step
por: Bruce, Emily A., et al.
Publicado: (2020)

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR
por: Song, Lele, et al.
Publicado: (2016)

Improving the standardization of mRNA measurement by RT-qPCR
por: Sanders, Rebecca, et al.
Publicado: (2018)

COVID-19 diagnosis by RT-qPCR in alternative specimens
por: Gonçalves, Cássia Cristina Alves, et al.
Publicado: (2021)

Rapid and accurate quantification of isomiRs by RT-qPCR
por: Franco, Sandra, et al.
Publicado: (2022)

Roadblock-qPCR: a simple and inexpensive strategy for targeted measurements of mRNA stability
por: Watson, Maegan J., et al.
Publicado: (2021)

Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion
por: Nelissen, Frank H. T., et al.
Publicado: (2009)

A novel method for the normalization of ChIP-qPCR data
por: Solomon, Elizabeth R, et al.
Publicado: (2021)

Evaluation of the mechanism of action of Bacillus spp. to manage Meloidogyne incognita with split root assay, RT-qPCR and qPCR
por: Gattoni, Kaitlin M., et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_acvp320njisjvr3jruqt34om1n